2011-12-01

Acoustophoresis offers non-contact and label-free cell separation based on size and intrinsic cell properties 14 without being detrimental to cells or altering their phenotype. 15 Indeed, Yang and colleagues demonstrated that microfluidic acoustophoresis could be used to effectively concentrate viable breast cancer cells (MCF7). 16 Osmotically induced cell death reduces the cell size and when

These results provide first evidence that NBCs can be acoustically separated from blood and stem cell preparations with high recovery and purity, thus indicating that acoustophoresis is a promising technology for the development of future label-free, non-contact cell processing of complex cell products. 2013-05-27 Acoustophoresis, the ability to acoustically manipulate particles and cells inside a microfluidic channel, is a critical enabling technology for cell-sorting applications. However, one of the major impediments for routine use of acoustophoresis at clinical laboratory has been the reliance on the inherent physical properties of cells for separation. Cancer cells display acoustic properties enabling acoustophoretic separation from white blood cells (WBCs) with 2–3 log suppression of the WBC background. However, a subset of WBCs has overlapping acoustic properties with cancer cells, which is why label-free acoustophoretic cancer cell isolation needs additional purification prior to analysis. Novel Cell Sorting and Separation Market: Focus on Acoustophoresis, Buoyancy-activated, Dielectrophoresis, Magnetophoretics, Microfluidics, Optoelectronics, Photoacoustics, Traceless Affinity and Other Technologies, 2019-2030 Novel Cell Sorting - Market research report and industry analysis - … 2016-02-26 2011-12-01 Hematopoietic progenitor cell assays revealed a preserved colony forming ability of nontarget cells post sorting. We conclude that the acoustophoresis platform can be utilized to efficiently isolate bead-labeled CD4+ lymphocytes from PBPC samples in a continuous flow format, with preserved functional capacity of both target and nontarget cells.

Cancer cells display acoustic properties enabling acoustophoretic separation from white blood cells (WBCs) with 2-3 log suppression of the WBC background. However, a subset of WBCs has overlapping acoustic properties with cancer cells, which is why label-free acoustophoretic cancer cell isolation needs additional purification prior to analysis. Label-free neuroblastoma cell separation from hematopoietic progenitor cell products using acoustophoresis – towards cell processing of complex biological samples. Franziska Olm, Anke Urbansky, Josefina H. Dykes, Thomas Laurell, and Stefan Scheding. US10640760B2 - Therapeutic cell washing, concentration, and separation utilizing acoustophoresis - Google Patents Therapeutic cell washing, concentration, and separation utilizing acoustophoresis Download PDF Info Publication number US10640760B2.

Chip integrated strategies for acoustic separation and manipulation of cells and particles · Free flow acoustophoresis: microfluidic-based mode of particle and cell

Free Flow Acoustophoresis (FFA) can be considered as one of the most efficient approaches for high-throughput separation of microparticles or cells from complex suspensions such as blood samples. 31,173,174 A dedicated review series has been written on the subject. 175–177 FFA uses acoustic forces generated by high Culture‐expanded mesenchymal stromal cells (MSCs) are promising candidates for clinical cell‐based therapies.

Label-free neuroblastoma cell separation from hematopoietic progenitor cell products using acoustophoresis – towards cell processing of complex biological samples. Franziska Olm, Anke Urbansky, Josefina H. Dykes, Thomas Laurell, and Stefan Scheding.

6 Mar 2016 which has broad implications in on-chip cytometry and cell sorting.

Novel Cell Sorting and Separation Market: Focus on Acoustophoresis, Buoyancy-activated, Dielectrophoresis, Magnetophoretics, Microfluidics, Optoelectronics, Photoacoustics, Traceless Affinity and Other Technologies, 2019-2030 Novel Cell Sorting - Market research report and industry analysis - 12665139 Acoustophoresis is a non-contact and label-free mode of manipulating particles and cell populations and allows for implementation of several separation modes . The technology is currently finding increased applications in bioanalytical and clinical applications of cell handling and manipulation. 2017-12-07 · Effective separation methods for fractionating blood components are needed for numerous diagnostic and research applications. This paper presents the use of acoustophoresis, an ultrasound based microfluidic separation technology, for label-free, gentle and continuous separation of mononuclear cells (MNCs) from diluted whole blood.
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We conclude that the acoustophoresis platform can be utilized to efficiently isolate bead-labeled CD4+ lymphocytes from PBPC samples in a continuous flow format, with preserved functional capacity of both target and nontarget cells.

Label-free neuroblastoma cell separation from hematopoietic progenitor cell products using acoustophoresis – towards cell processing of complex biological samples. Franziska Olm, Anke Urbansky, Josefina H. Dykes, Thomas Laurell, and Stefan Scheding.
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separated from red blood cells (RBCs) shows a purity of more than 96%. Petersson K, Augustsson P and Laurell T 2013 Acoustophoresis Separation of.

ACOUSTOPHORESIS SEPARATION OF BACTERIA FROM BLOOD CELLS FOR RAPID SEPSIS DIAGNOSTICS P. D. Ohlsson*, K. Petersson, P. Augustsson and T. Laurell Lund University, SWEDEN ABSTRACT We present a significantly improved acoustophoresis method to separate bacteria from red and white blood cells. As Cancer cells display acoustic properties enabling acoustophoretic separation from white blood cells (WBCs) with 2–3 log suppression of the WBC background. However, a subset of WBCs has overlapping acoustic properties with cancer cells, which is why label-free acoustophoretic cancer cell isolation needs additional purification prior to analysis. New students International Desk Academic matters & support IT services & support Careers Service Study abroad opportunities Become an international mentor Represent & promote LU Health care Financial matters LU Accommodation tenants Options for learning Swedish Current doctoral students When leaving LU and Sweden Coronavirus – info for students Acoustophoresis has gained increasing attention as a gentle, non-contact, and high-throughput cell and particle separation technique. It is conveniently used to isolate and enrich particles that are greater than 2 μm; however, its use in manipulating particles smaller than 2 μm is limited. In this work, we present an alternative way of using acoustic forces to manipulate sub-micrometer Acoustophoresis Enables the Label-Free Separation of Functionally Different Subsets of Cultured Bone Marrow Stromal Cells Franziska Olm,1 Hooi Ching Lim,1 Katharina Schallmoser,2 Dirk Strunk,3 Thomas Laurell,4 Stefan Scheding1,5* Abstract Culture-expanded mesenchymal stromal cells (MSCs) are promising candidates for clini-cal cell-based therapies.

Culture‐expanded mesenchymal stromal cells (MSCs) are promising candidates for clinical cell‐based therapies. MSC products are heterogeneous and we therefore investigated whether acoustophoresis, an ultrasound‐based separation technology, could be used for the label‐free enrichment of functionally different MSC populations.

Att separera celler i mikrochip med hjälp av ultraljud är även en skonsam metod så cellerna klarar sig bra efteråt, vilket är en av anledningarna till att den är intressant. Detta arbete var en start i att försöka sortera ut vissa celler från upplöst och utspädd vävnad och har visat lovande resultat som kommer att forskas vidare på i gruppen. TY - JOUR. T1 - Free flow acoustophoresis: Microfluidic-based mode of particle and cell separation. AU - Petersson, Filip. AU - Aberg, Lena.

As Cancer cells display acoustic properties enabling acoustophoretic separation from white blood cells (WBCs) with 2–3 log suppression of the WBC background. However, a subset of WBCs has overlapping acoustic properties with cancer cells, which is why label-free acoustophoretic cancer cell isolation needs additional purification prior to analysis. New students International Desk Academic matters & support IT services & support Careers Service Study abroad opportunities Become an international mentor Represent & promote LU Health care Financial matters LU Accommodation tenants Options for learning Swedish Current doctoral students When leaving LU and Sweden Coronavirus – info for students Acoustophoresis has gained increasing attention as a gentle, non-contact, and high-throughput cell and particle separation technique. It is conveniently used to isolate and enrich particles that are greater than 2 μm; however, its use in manipulating particles smaller than 2 μm is limited. In this work, we present an alternative way of using acoustic forces to manipulate sub-micrometer Acoustophoresis Enables the Label-Free Separation of Functionally Different Subsets of Cultured Bone Marrow Stromal Cells Franziska Olm,1 Hooi Ching Lim,1 Katharina Schallmoser,2 Dirk Strunk,3 Thomas Laurell,4 Stefan Scheding1,5* Abstract Culture-expanded mesenchymal stromal cells (MSCs) are promising candidates for clini-cal cell-based therapies. We present a new experimental method that measures the compressibility of phenotype-specific cell populations. This is done by performing statistical analysis of the cell counts from the outlets of an acoustophoresis chip as a function of the increasing actuator voltage (i.e.